Question: Need help with "Barcode Splitter" tool
gravatar for tymiller
4.0 years ago by
United States
tymiller60 wrote:


I would like to use the new "enhanced" barcode splitter that was developed this past year. It is in the Galaxy Main toolshed, but not available to use in workflows on Galaxy Main.  It is basically the same thing, but gives the option to directly import the output files to a new history, preventing the user from having to download and upload each file individually (very helpful when looking at multiplexed data with 45 barcodes, like I am doing now).

Would it be possible to add this tool in place or in addition to the Barcode Splitter tool now available on Galaxy Main? Searching through BioStar, this seems like a frequent question and would be helpful for many researchers! It can be found here:

In addition, ideally, there would be a way to output the split files directly into the next step in the workflow. Is there anyway to do this?  For example, if someone had 10 files coming from the Barcode Splitter step in the workflow, is there a way to send those files directly into the next step, for example a Trim step?




Tool name: Barcode Splitter
Tool version: 1.0.0
Tool ID:
ToolShed URL:


barcode-splitter • 1.3k views
ADD COMMENTlink modified 4.0 years ago by Lance Parsons110 • written 4.0 years ago by tymiller60
gravatar for Lance Parsons
4.0 years ago by
Lance Parsons110
United States
Lance Parsons110 wrote:

I wrote the enhanced version of the barcode splitter tool to meet our own institutional needs, but I'm glad you find the enhancements useful.  I can't speak to the plans of the Galaxy team to install things like that on Main, however, I've had similar frustrations about using the tool in a workflow. Since the tool was written, there have been many enhancements to the tool and workflow framework including the ability to run workflows on multiple files, etc.  Does anyone on the Galaxy team have any insight into how we might be able to modify the tool to allow it to be used in workflows?  The main issue that the number of output files depends on the number of barcodes in one of the input files and thus is not known apriori (but could be determined easily and quickly).

ADD COMMENTlink written 4.0 years ago by Lance Parsons110

Looks like this Trello card ( covers functionality that should make this possible.  I'll track it and try to get the tool updated once the new functionality is released.

ADD REPLYlink written 4.0 years ago by Lance Parsons110

I'm working on a project that would really benefit from using the barcode splitter tool in its workflow as well - do you happen to know if this is available yet?

ADD REPLYlink written 3.4 years ago by mccoykg80

As far as I know, its still not available. I ended up just copying all the links into excel, using a macros to display the entire link url and then copy and pasting them into the "Get Data" "Upload". Not perfect but relatively efficient

ADD REPLYlink written 3.4 years ago by tymiller60

Thanks, I'll try that!

ADD REPLYlink written 3.4 years ago by mccoykg80

I used visual basic and this was code Sub ExtractHL() Dim HL As Hyperlink For Each HL In ActiveSheet.Hyperlinks HL.Range.Offset(0, 1).Value = HL.Address Next End Sub

ADD REPLYlink written 3.4 years ago by tymiller60
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 174 users visited in the last hour