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Question: Need help with "Barcode Splitter" tool
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gravatar for Nicole.Francis
4.4 years ago by
Nicole.Francis • 20
Canada
Nicole.Francis • 20 wrote:

i uploaded a fastq file of the output from the sequencing but it is not recognized as a "library" in the barcode splitter tool. this is an example of the format of the reads:

+
@CCDDDDDHHHHHGCCCGIGIIJJJDHECGGIGHIJ@FFHHJJIIJJJJJJJJIFHIHEHFFFFDCEDCCECCB##########################
@HWI-ST865:343:C3UTDACXX:3:1101:1493:2000 1:N:0:
CTGATCGTTGATGGTTCGGGGTATTATCTATGAGTATAGAGATCGTAGTTCAGCCCAAGTGTTACACTGTTGTAGCTGGGATACTTCTTGTGCATGGCCA
+

 

Tool name: Barcode Splitter
Tool version: 1.0.0
Tool ID: toolshed.g2.bx.psu.edu/repos/devteam/fastx_barcode_splitter/cshl_fastx_barcode_splitter/1.0.0
ToolShed URL: http://toolshed.g2.bx.psu.edu/view/devteam/fastx_barcode_splitter

 
barcode-splitter • 1.1k views
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modified 4.4 years ago • written 4.4 years ago by Nicole.Francis • 20
2
gravatar for Nicole.Francis
4.4 years ago by
Nicole.Francis • 20
Canada
Nicole.Francis • 20 wrote:

answer available on galaxy wiki.

https://wiki.galaxyproject.org/Support#FASTQ_Datatype_QA
ADD COMMENT • link written 4.4 years ago by Nicole.Francis • 20
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