3.9 years ago by
You are working on the public Main Galaxy instance at http://usegalaxy.org?
If so, choose a Reference genome from the pull-down list that is the exact same genome as used when mapping the sequences to produce the BAM alignment dataset. And if the genome is not available, you can upload a fasta version of the genome you need and use it as Custom Reference genome.
Help to determine if the genomes are a match, and for Custom Genomes:
If instead you are working on a local or cloud Galaxy that you administrator over, a genome can be added directly or as a Custom genome. More is in our admin help:
Hopefully this helps, Jen, Galaxy team