Question: Barcode limit in "Barcode Splitter" tool
gravatar for alberto.gil.12
4.0 years ago by
United Kingdom
alberto.gil.1210 wrote:


I was wondering what is the barcode limit to be run in the "Barcode splitter" tool.

I have successfully run it 50 barcodes but I have libraries with 1000s of barcodes and an error message like this came up.

Error: failed to create output file (/galaxy/main/jobdir/008/270/8270026/dataset_9798426_files/libraryfasta_fa__138172.txt)

Thank you, 



Tool name: Barcode Splitter
Tool version: 1.0.0
Tool ID:
ToolShed URL:


barcode-splitter • 1.3k views
ADD COMMENTlink modified 4.0 years ago by Jennifer Hillman Jackson25k • written 4.0 years ago by alberto.gil.1210
gravatar for Jennifer Hillman Jackson
4.0 years ago by
United States
Jennifer Hillman Jackson25k wrote:


I am not aware of any specific limit, but the job may simply be running out of memory. Try using less barcodes in one single run. You can re-run with more on the results that are not recognized/sorted by the prior runs.

Hopefully this works out for you,

Thanks, Jen, Galaxy team

ADD COMMENTlink written 4.0 years ago by Jennifer Hillman Jackson25k
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