Question: Barcode limit in "Barcode Splitter" tool
1
gravatar for alberto.gil.12
4.0 years ago by
United Kingdom
alberto.gil.1210 wrote:

Hello, 

I was wondering what is the barcode limit to be run in the "Barcode splitter" tool.

I have successfully run it 50 barcodes but I have libraries with 1000s of barcodes and an error message like this came up.

Error: failed to create output file (/galaxy/main/jobdir/008/270/8270026/dataset_9798426_files/libraryfasta_fa__138172.txt)

Thank you, 

Albert


 

Tool name: Barcode Splitter
Tool version: 1.0.0
Tool ID: toolshed.g2.bx.psu.edu/repos/devteam/fastx_barcode_splitter/cshl_fastx_barcode_splitter/1.0.0
ToolShed URL: https://toolshed.g2.bx.psu.edu/view/devteam/fastx_barcode_splitter

 

barcode-splitter • 1.3k views
ADD COMMENTlink modified 4.0 years ago by Jennifer Hillman Jackson25k • written 4.0 years ago by alberto.gil.1210
0
gravatar for Jennifer Hillman Jackson
4.0 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

I am not aware of any specific limit, but the job may simply be running out of memory. Try using less barcodes in one single run. You can re-run with more on the results that are not recognized/sorted by the prior runs.

Hopefully this works out for you,

Thanks, Jen, Galaxy team

ADD COMMENTlink written 4.0 years ago by Jennifer Hillman Jackson25k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 177 users visited in the last hour