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Question: 16S sequencing analysis
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gravatar for meressah
3.8 years ago by
meressah • 0
United States
meressah • 0 wrote:

I would like to know if it possible to use data from Next-gen platforms for 16S rRNA sequencing can be  analyze with usegalaxy software and if so how.
mothur 16s metagenomics galaxy • 1.7k views
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modified 3.8 years ago by Jennifer Hillman Jackson ♦ 25k • written 3.8 years ago by meressah • 0
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gravatar for Jennifer Hillman Jackson
3.8 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

There are several wrapped tools for rRNA analysis in the Tool Shed and any other tool that you need could be wrapped for Galaxy (if you have that resource available). These tools are for use in a local/cloud Galaxy and there are Public Galaxy instances that host tools for this analysis. Below is also a link for a tutorial linked to some of these tools. Other publications/protocols can be reviewed from any source and compared to the tools Galaxy has wrapped already.

Search using the keyword 'rRNA' to locate tools:
http://usegalaxy.org/toolshed

Server choices:
http://wiki.galaxyproject.org/PublicGalaxyServers

In particular, please see what the GVL offers in the form of tutorials and the related tools. This will help you to decide how to set up your own cloud Galaxy if you decide to use their wrapped tools:
http://wiki.galaxyproject.org/PublicGalaxyServers#GVL_Tutorial
http://genome.edu.au/wiki/Learn

Hopefully this helps to get you started, Jen, Galaxy team
ADD COMMENT • link written 3.8 years ago by Jennifer Hillman Jackson ♦ 25k
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