Question: Re: Simulating Sequencing And Removing Redundant Sequences
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Kevin Lam • 50 wrote:
Hi Daniel
for 2) you may use the tools under NGS QC and manipulation
FASTQ to FASTA<http: main.g2.bx.psu.edu="" tool_runner?tool_id="cshl_fast" q_to_fasta="">converter
followed by
Collapse<http: main.g2.bx.psu.edu="" tool_runner?tool_id="cshl_fastx_col" lapser="">sequences
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Kevin Lam • 50 wrote:
Hi Daniel,
You would have multiple names for each sequence and that would be
quite hard
to display. I am sure someone thought through this. Since the sequence
is
the same, you can use the sequence to look back in the fastq file for
read
name. Although I am not sure how that would help you?
Cheers
Kevin
For read simulation, you may also want to give Grinder a try. I made a
Galaxy wrapper for it (see in the toolshed:
http://toolshed.g2.bx.psu.edu/)
Florent
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