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• Need help with "Filter SAM" tool
      Tool name: Filter SAM Tool version: 1.0.0 Tool ID: toolshed.g2.bx.psu.edu/repos/devteam/sa...
• Need help with "Filter by quality" tool
  which formet this tool gets?   Tool name: Filter by quality Tool version: 1.0.0 Tool ID: tools...
• Need help with "Filter by quality" tool
  Why doesn't this tool recognize my fastq files? It won't let me run the tool on the fastq files I...
• Need help with "Principal Component Analysis" tool
  I want to convert DNA sequence into RNA sequence how to upload target DNA sequence. it is saying...
• Need help with "Principal Component Analysis" tool
      Tool name: Principal Component Analysis Tool version: 1.0.2 Tool ID: toolshed.g2.bx.psu.ed...
• Need help with "FASTQ Groomer" tool
  Hi, I try to groom a sequence from NCBI sequenced from ilumina Hiseq 2000 by BGI, it's running...
• Need help with "Trim sequences" tool
  is it normal it does not work on fastq files? I had to convert my fastq into fasta in order to t...
• Need help with "Barcode Splitter" tool
  i uploaded a fastq file of the output from the sequencing but it is not recognized as a "library"...
• Need help with "FASTQ Groomer" tool
  Hi, How do I upload if I have 2 files for paired-end sequence?   Thanks, Tina   Tool name:...
• Need help with "Fetch taxonomic representation" tool
  Hello, i need to fetch the HCV sequence from my data set how can i use the fetch-taxonomic-repre...
• Need help with "Variant Annotator" tool
    What is the meaning of this error" History does not include a dataset of the required format /...
• Need help with "FASTQ Quality Trimmer" tool
  I am not able to add my file to this tool.     Tool name: FASTQ Quality Trimmer Tool version:...
• Need help with "FASTQ Trimmer" tool
  FASTQ Trimmer seems unable to recognize my FASTQ files as input. For e.g. I carried out FASTQ to ...
• Need help with "Unified Genotyper" tool
  Every time I try to use the Unified Genotyper Tool under GATK, I receive the following error mess...
• Need help with "Map with BWA for Illumina" tool
   I don't know why the mapping procedure doesn't work.  My g-DNA sequence is fatsaq format, it can...
• Need help with "FastQC:Read QC" tool
  I have collected the raw data for my RNA-seq project. This dataset includes short DNA sequences o...
• Need help with "Clip" tool
  hi, i have a question about this tool i heard that galaxy has 'trimmomatic' tool. But i can't f...
• Need help with "Tophat2" tool
  I have uploaded fastq file to the galaxy, I can see it on the right "history", but I can not see ...
• Need help with "Filter pileup" tool: not recognizing pileup data in "Select Dataset" popup menu
  I have been (roughly) following the tutorial on https://usegalaxy.org/u/aun1/p/mtdemo-getting-th...
• Need help with "Generate pileup" tool
  Dear, What kind of files should i use to "using reference genome" and where get it? I trying to...
• Setting clipping sequence as workflow parameter when running FASTX Clipper from BioBlend API
  I am using the BioBlend API to run a workflow on Galaxy, and I am having trouble setting the para...
• Need help with "FASTQ Quality Trimmer" tool
  I have uploaded .fastq files successfully but the "FASTQ file" drop-down list doesn't show my .fa...
• Need help with "patmatdb" tool
  Everytime I try to use open brackets or "{ or }" I get an error message.  Would really like to be...
• Need help with "Mark Duplicate reads" tool
  What is the aim of this tool?   Tool name: Mark Duplicate reads Tool version: 1.56.0 Tool ID: ...