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Question: Need help with "Tophat for Illumina" tool
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gravatar for 52133490
3.9 years ago by
52133490 • 0
European Union
52133490 • 0 wrote:

I do not know why: "RNA-Seq FASTQ file:" can not been selected? 

 

 

Tool name: Tophat for Illumina
Tool version: 1.5.0
Tool ID: toolshed.g2.bx.psu.edu/repos/devteam/tophat/tophat/1.5.0
ToolShed URL: https://toolshed.g2.bx.psu.edu/view/devteam/tophat

 
tophat-for-illumina • 803 views
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modified 3.9 years ago by Jennifer Hillman Jackson ♦ 25k • written 3.9 years ago by 52133490 • 0
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gravatar for Jennifer Hillman Jackson
3.9 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Double check that your inputs are both in the correct format (content-wise, in particular quality score scaling) and are assigned the proper datatype attribute (.fastqsanger). It is very important that both are true (an improperly assigned datatype will just lead to problems later on - meaning that you will have to start over!).

Exact how-to help is here in our wiki:
http://wiki.galaxyproject.org/Support#FASTQ_Datatype_QA

More help for similar questions is also in prior Q&A - try a search here if you wish to read more details.

Best, Jen, Galaxy team
ADD COMMENT • link written 3.9 years ago by Jennifer Hillman Jackson ♦ 25k
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