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• Analysis workflow of miRNA-seq data
  I'm looking to analyse RNA-Seq data from miRNA. How similar is the workflow to RNA-seq analysis?
• How to get a hg38 miRNA annotation file work in galaxy?
  Hi, I just started to use galaxy to analyze our NGS data, so I'm not good at it. My purpose is t...
• Workflow to clean data prior to variant call
  Hi,  I would like some advice on the workflow I'm using. 1- I have WGS data from bacteria, I've...
• Uploading Gz Files
  I am unable to load the raw alignment data from the broad institute datasets available from the u...
• Mapping Mirna Data
  Dear All I am interested in using Galaxy to compare miRNA sequencing data. I have previously gr...
• How to get raw reads from bam file
  Hi, I can't figure out how to get raw reads counts from a bam file. I have 3 different experimen...
• interogation of micro rna in livercirhosis using ngs data (galaxy software)
  hi there I was using galaxy software online for alighning my mirna seq data . i have a queasion...
• VERY new to Galaxy: How do I query by row and keep rows with specific values
  Hi - I've been using Galaxy for about a week now on my own. I'm a total newcomer this world, so p...
• miRNA sequencing alignment
  i have a rna-seq data and would like to align it against micro RNA human. Can I do it in galaxy. ...
• Mirna Targets At All Transcribed But Not Translated Sites At Genome Level
  I have an intuitive feeling, It will be possible to do it in Galaxy, to be precise: I want to f...
• Cannot Get Rid Of Custom Build Error
  Hello, I've tried to create a custom build (starting from the trackster page) and clearly made s...
• Reducing Size On Disk To Comply With Quotas
  Hi, I am currently using 636gb of storage and would like to reduce it. I have deleted (from disk)...
• Normalization of RNA-seq data
  Hi, I am wanting to normalize my RNA-seq data without running a differential gene expression on...
• Over disk quota issue!
  Hi, I am getting the error message that I am over my disk quota- I have deleted a lot of data si...
• Encountered message from UCSC: "Reached output limit of 100000 data values"
  Hello, I am a green hand in using Galaxy. I have got two tables from UCSC, one is about the miR...
• DE analysis of miRNA after mapping with miRDeep2 quantifier
  After small RNA sequencing I performed adapter, quality, and length trimming on fastq files. I th...
• Issues with Using CuffDiff Tool in Galaxy
  Hi,   I am analyzing microRNASeq data. My pipeline is qc reads, bowtie for alignment, cufflinks...
• Error in CuffDiff
  I would be forever grateful if someone could take the time to help me. I am attempting to analyze...
• How to obtain more space for raw data and analysis
  Hi I will use galaxy for RNA-seq analysis I start to upload the raw data FatQ file I will reach t...
• raw data (23andme or Ancestry) free access for research
  Hello to all, I am doing genetic tests (AncestryDNA and 23andme) on me and my family and I will h...
• Raw Count Of Genes
  Dear all, I am a Phd student working on chicken genomics, with limited experience in the bio-inf...
• Mate_Not_Found after FixMate and filter
  Hi After applying FixMate and filtering on "isMapped" and "isMatemapped" (and quality and no dup...
• Analysis Of 454 Data
  Dear All, I'm very very much new to this ngs platform... and I'm currently getting started to w...
• RNA-seq raw data of zebrafish
  Hello! I need RNA-seq raw data of tumors in zebrafish for a PhD job, does anyone knows an useful...