Question: Raw Count Of Genes
gravatar for Els Willems
5.6 years ago by
Els Willems30
Els Willems30 wrote:
Dear all, I am a Phd student working on chicken genomics, with limited experience in the bio-informatics field. I performed an RNA-Seq experiment with single end 50 bp reads to find differential gene expressions between different groups. I have mapped this data with Tophat. Does anyone know how I can get the raw data count out of galaxy in a tab-delimited file? I would like to perform a statistical analysis in other software, without the RPKM normalization that cufflinks offers. Thank you very much. Have a nice day. Yours Sincerely, Els Ir. Els Willems KU Leuven Department of Biosystems Division Livestock - Nutrition - Quality Laboratory of Livestock Physiology Kasteelpark Arenberg 30 bus 2456 B - 3001 Heverlee T (+32) 016 32 17 29 F (+32) 016 32 19 94
rna-seq cufflinks • 1.2k views
ADD COMMENTlink modified 5.6 years ago by Björn Grüning350 • written 5.6 years ago by Els Willems30
gravatar for Björn Grüning
5.6 years ago by
Björn Grüning350 wrote:
Hi Els, try the tool 'Group data by a column and perform aggregate operation on other columns.' And choose 'count' or 'count distinct. Hope that will work. Happy research! Bjoern
ADD COMMENTlink written 5.6 years ago by Björn Grüning350
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 169 users visited in the last hour