Question: Unicycler assembly on galaxy
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gravatar for hopeserepa
9 months ago by
hopeserepa • 0
hopeserepa • 0 wrote:

Hi

I have a bacterial genome (illumina) which I have to assemble. On trimming the data with Trimmomatic, i get errors when I assemble with Unicycler. But when I assemble the data without trimming it gets assembled. Anyone know what might be wrong? Please assist.

Hope

ADD COMMENT • link • modified 9 months ago by Jennifer Hillman Jackson ♦ 25k • written 9 months ago by hopeserepa • 0
0
gravatar for Jennifer Hillman Jackson
9 months ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The reads are probably getting trimmed down too much, creating shorter sequences that do not assemble well. Run FastQC on the data before trimming and after and review. You might need to adjust the trim settings, use a different trim tool, or skip trimming altogether.

Galaxy tutorials

Thanks! Jen, Galaxy team

ADD COMMENT • link written 9 months ago by Jennifer Hillman Jackson ♦ 25k
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