Question: Contig assembly on MicrobesNG data
gravatar for robin.hoeven
6 months ago by
robin.hoeven0 wrote:


I recently had the genome of the bacteria that I am working on sequenced by MicrobesNG. The problem is that I don't know how to convert the contig data into a full genome sequence. I tried using Unicycler on the galaxy website, but the run always comes back with a "failure preparing job" error.

I've uploaded the fasta file that MicrobesNG provided me with, which looks as follows:


for the settings in Unicycler, I've tried "single" end data, with dragging the fasta file to the "unpaired reads" location, and I also select this fasta file in the "long reads" section. Otherwise I haven't changed any of the settings and just pressed "execute".

Does anyone know what I am doing wrong and how to fix it? I don't know if the data that I have is in an unconventional format and does it therefore need some preprocessing before I can submit it to Unicycler?

Thank you

ADD COMMENTlink written 6 months ago by robin.hoeven0
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