Hello, I have a paired-end 150 bp genome sequencing data with a different end quality.According to the fastQC analysis, the 10 last nucleotides of one read should be trimmed, while for the other one 30 nucleotides need to be trimmed. Can I trim different numbers of nucleotides from each read and then assemble them? Or, as it paired-end it should be same? Regards, Aida
Please see the Galaxy Assembly tutorials here for an overview of how to use the tools (with more details linked from the underlying tool's manuals): http://galaxyproject.github.io/training-material/topics/assembly/
In short, some tools expect a consistent insert size/sequence lengths and others are more flexible.
All Galaxy tutorials: https://galaxyproject.org/learn/
Thanks! Jen, Galaxy team