Question: Quality Assessment of RNA de novo assemble (trinity) without reference genome
5 months ago by
sdbaney • 10
sdbaney • 10 wrote:
Hello, Are there any tools within the galaxy tool shed that would give me a quality assessment of my trinity assembly without using a reference genome (as there is none for my organism)? I ran two assemblies with the same data trimmed with different parameters and I am trying to figure out a way to determine which one yielded a better assembly. Unfortunately, all of the quality assessment tools uses a reference genome to map the transcripts back to it which I don't have.