Question: Unicycler assembly on galaxy
0
gravatar for hopeserepa
6 months ago by
hopeserepa0
hopeserepa0 wrote:

Hi

I have a bacterial genome (illumina) which I have to assemble. On trimming the data with Trimmomatic, i get errors when I assemble with Unicycler. But when I assemble the data without trimming it gets assembled. Anyone know what might be wrong? Please assist.

Hope

ADD COMMENTlink modified 6 months ago by Jennifer Hillman Jackson25k • written 6 months ago by hopeserepa0
0
gravatar for Jennifer Hillman Jackson
6 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

The reads are probably getting trimmed down too much, creating shorter sequences that do not assemble well. Run FastQC on the data before trimming and after and review. You might need to adjust the trim settings, use a different trim tool, or skip trimming altogether.

Galaxy tutorials

Thanks! Jen, Galaxy team

ADD COMMENTlink written 6 months ago by Jennifer Hillman Jackson25k
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