I had a mutant and wild type FASTQ data of a mutant yeast strain. How can I perform FASTQ quality trimmer to get quality for each base > 10? How to identify mutations in mutant file which are not in wild type?
FastQC > Trimmomatic is a common choice for QA.
Galaxy tutorials for variant analysis are here: https://galaxyproject.org/learn/. Please also see: https://biostar.usegalaxy.org/p/26130/#26140
Thanks! Jen, Galaxy team