Question: Should I Run Fastq Groomer?
0
gravatar for Du, Jianguang
6.3 years ago by
Du, Jianguang380
Du, Jianguang380 wrote:
Dear All, I have some FASTQ datasets in phred 33 offset, and I have already assinged them Fastqsanger format. Do I need to run FASTQ Groomer on these datasets before I check the data quality by "Fastqc: Fastqc QC" and "FASTQ Trimmer by column" to remove bad nucleotides at 3' end of reads? Should I select "Sanger" as "Input FASTQ quality scores type:" if I need to run Groomer? Thanks. Jianguang Du
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ADD COMMENTlink modified 6.3 years ago by Jennifer Hillman Jackson25k • written 6.3 years ago by Du, Jianguang380
0
gravatar for Jennifer Hillman Jackson
6.3 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello Jianguang, Given this information, you will not need to run FASTQ Groomer, but simply proceed with Fastqc and FASTQ Trimmer. Best, Jen Galaxy team -- Jennifer Jackson http://galaxyproject.org
ADD COMMENTlink written 6.3 years ago by Jennifer Hillman Jackson25k
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