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Question: how to convert FASTQ to 454 reads
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gravatar for Kazi Shawrob
20 months ago by
Kazi Shawrob • 0
University of Science and Technology Chittagong , Bangladesh.
Kazi Shawrob • 0 wrote:

I am totally new at usegalaxy.org. I didn't know the difference between FASTQ reads and 454 reads. I wanted to run a workflow on meta genomics. It wants 454 reads, but I have FASTQ sequences and I don't know much about 454 reads, so how can I convert FASTQ to 454 sequences. I am sorry for my bad English
fastq 454 reads metagenomics • 445 views
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modified 19 months ago by Jennifer Hillman Jackson ♦ 25k • written 20 months ago by Kazi Shawrob • 0
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gravatar for Jennifer Hillman Jackson
19 months ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The majority of Galaxy tools require .fastqsanger or .fastqsanger.gz formatted sequence data. Some accept simply .fastq or .fastq.gz, but eventually, for almost all downstream tools, fastqsanger format will be needed, as explained here: https://galaxyproject.org/support/fastqsanger/

If you are just asking if Galaxy can do this conversion and later plan to use tools outside of Galaxy, then using a standalone (line-command) tool to convert might be a better approach. I personally cannot recommend one, but I see that you asked the same question here, which is the correct way forward for help: https://biostar.usegalaxy.org/p/22500/

Thanks, Jen, Galaxy team
ADD COMMENT • link written 19 months ago by Jennifer Hillman Jackson ♦ 25k
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