Question: how to convert FASTQ to 454 reads
gravatar for Kazi Shawrob
20 months ago by
University of Science and Technology Chittagong , Bangladesh.
Kazi Shawrob0 wrote:

I am totally new at I didn't know the difference between FASTQ reads and 454 reads. I wanted to run a workflow on meta genomics. It wants 454 reads, but I have FASTQ sequences and I don't know much about 454 reads, so how can I convert FASTQ to 454 sequences. I am sorry for my bad English

fastq 454 reads metagenomics • 445 views
ADD COMMENTlink modified 19 months ago by Jennifer Hillman Jackson25k • written 20 months ago by Kazi Shawrob0
gravatar for Jennifer Hillman Jackson
19 months ago by
United States
Jennifer Hillman Jackson25k wrote:


The majority of Galaxy tools require .fastqsanger or .fastqsanger.gz formatted sequence data. Some accept simply .fastq or .fastq.gz, but eventually, for almost all downstream tools, fastqsanger format will be needed, as explained here:

If you are just asking if Galaxy can do this conversion and later plan to use tools outside of Galaxy, then using a standalone (line-command) tool to convert might be a better approach. I personally cannot recommend one, but I see that you asked the same question here, which is the correct way forward for help:

Thanks, Jen, Galaxy team

ADD COMMENTlink written 19 months ago by Jennifer Hillman Jackson25k
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