Question: Convert fna/.qual files to .fastq
gravatar for oreillys
4.1 years ago by
United States
oreillys0 wrote:

I have 454 .fna and .qual files that I would like to convert to fastq format. It appears that only the reverse conversion can be done with Galaxy.

Could someone advise if this can be done, and if so how?

Thank you

galaxy • 3.1k views
ADD COMMENTlink modified 4.1 years ago by Jennifer Hillman Jackson25k • written 4.1 years ago by oreillys0
gravatar for Jennifer Hillman Jackson
4.1 years ago by
United States
Jennifer Hillman Jackson25k wrote:


Please see the tools under "NGS: QC and manipulation". You will need the dataset assigned to a .fastqsanger "datatype" before using it with most tools (scale the quality scores to Sanger Phred+33).

  • Combine FASTA and QUAL - merge
  • Fastq Groomer - scale quality scores, assign datatype

This tool section has been reorganized. Any tools that were in this location previously, but that are no longer supported on the public Main Galaxy instance (, can be found in the Tool Shed ( for use in a local or cloud Galaxy (

Thanks! Jen, Galaxy team

ADD COMMENTlink written 4.1 years ago by Jennifer Hillman Jackson25k
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