Question: extracting sequence from E. coli genomic sequences
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kajohnso • 0 wrote:
In the lab I conjugated a bacteria of interest with E. coli and was able to transfer an antibiotic resistance gene. I do not know what this gene is. I have tried molecular approaches to determining the identity of the gene without success. I have now sequenced the conjugated E. coli with the resistance gene and have a fastq file. I am looking for advise in how to 'subtract' the E. coli sequence and leave the transferred sequence(s). I have tried lastZ, but get short reads that don't give me much information (usually below 150 bp).
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modified 2.5 years ago
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Jennifer Hillman Jackson ♦ 25k
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2.5 years ago by
kajohnso • 0
