Question: Analysis 454 Quality Data
0
Elad Firnberg • 20 wrote:
Hi,
I am starting off with 454 read data in an sff file. I would like to
get
quality statistics on the data, but having trouble getting the tools
to
work.
I first tried to convert to a fastq file and use the "Compute Quality
Statistics" tool, but I get this error, "An error occurred running
this job:
*fastx_quality_stats: found invalid nucleotide sequence "*
I then tried the "fastq groomer" and repeated the "Compute Quality
Statistics", but got the same error. Perhaps it cannot handle the
longer
454 sequences?
*
*
Alternatively I tried converting the sff file to a fasta file and
quality
file. I had to manually convert the quality data file to qual454 for
the
"Build Base Quality Distribution" tool to recognize it, but upon doing
that
I got this error: *"*An error occurred setting the metadata for this
dataset." And the Build Base Quality Distribution tool, also failed.
*
*
*
*
Any help resolving this issue would be appreciated,
Thank you,
Elad
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modified 6.8 years ago
by
Jennifer Hillman Jackson ♦ 25k
•
written
6.8 years ago by
Elad Firnberg • 20
