Question: Bedgraph to bigwig generates error
1
djevo1 • 60 wrote:
I am running bedgraph to bigwig on a local galaxy and am receiving this error. The bedgraph files are set to the correct genome build and the .len file has been created.
Fatal error: Matched on Error
Error running wigToBigWig.
There is likely an admin configuration error, if you are certain the genomes are an exact match and the genome fasta itself has been added to the local and indexed for SAMtools, Picard, and 2bit (recommended for all genomes to be used as a baseline, add in other indexes as wanted).
There could be other related factors (job uses more resources than available on the local - specifically memory), but the test below can help determine that and help with further troubleshooting.
One way to test is to see if the jobs work at http://usegalaxy.org. Use a Custom reference genome "Build" if needed. A custom genome build could also be used on your local, but less resources will be used if installed/indexed.
All support FAQs: https://galaxyproject.org/support/
All Galaxy tutorials: https://galaxyproject.org/learn/
Thanks, Jen, Galaxy team
I used the db keys all fasta to generate the len file, inedexed for picard, samtools, 2bit. I may know what it is however. Is there a way to generate a len file from multiple fasta files, one for each chromosome?
Finally got around to uploading the len file to usegalaxy, I receive the same error. I was using one of my bam files as well.
hashMustFindVal: 'GL000192.1' not found