I have a very large, merged BAM file which I successfully converted to a bedgraph using the "Create a BedGraph of genome coverage" tool. I was then trying to convert this file to bigwig format (to aid viewing with IGV) using the Wig/BedGraph-to-bigWig tool with the default settings. However, I get the following error:
hashMustFindVal: '1' not found Error running wigToBigWig. Error running wigToBigWig.
I used the same (custom) genome assembly for the whole process, and the correct genome is applied in the attributes section of the input file.
Any help or ideas would be much appreciated!