Hi, I am new to the world of NGS bioinformatics. I am not very good with command based programs. I have an RNASeq dataset for 49 samples (paired-end reads). I am wondering if I can do the analyses using the web-based platform if I upload all the data through FTP. I have almost 130 Gb of data. Any help and guidance will be greatly appreciated.
Galaxy's web based tools can be used for RNA-seq analysis. This tutorial provides an overview: https://github.com/nekrut/galaxy/wiki/Reference-based-RNA-seq
Your data size may require the use of a local or cloud Galaxy. Please see: https://wiki.galaxyproject.org/BigPicture/Choices
For Support/Help resources, the Galaxy Wiki is a good place to find information: https://wiki.galaxyproject.org/Support
Best, Jen, Galaxy team