I used functions of “Create a BedGraph of genome coverage” and “Wig/BedGraph-to-bigWig” in Galaxy to convert a BAM file into a bigwig file. It worked well when BAM files were aligned onto a chicken galGal4 reference genome downloaded from the UCSC Genome Browser. However, it doesn’t work when the chicken reference genome were downloaded from Ensembl (Release 72 or 75). The below is the error message from “Wig/BedGraph-to-bigWig”. Could you help me? Thanks a lot.
An error occurred with this dataset:
hashMustFindVal: '1' not found
Error running wigToBigWig.