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Question: Please Help Rmdupl
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gravatar for Teresita Diaz De Ståhl
6.6 years ago by
Teresita Diaz De Ståhl • 10
Teresita Diaz De Ståhl • 10 wrote:
Hello I wonder if it is possible to rmdupl using galaxy on the free public server. I uploaded the bam files via ftp main.g2.bx.psu.edu) but they are not visible in my public interface, I can only see the fastq uploaded files. I would very much appreciate some suggestion how to remove PCR duplicates from my bam files Thanks Teresita Teresita Díaz de Stĺhl, Department of Oncology-Pathology Cancer Center Karolinska, CCK R8:04 Karolinska Institutet SE-171 76 Stockholm, Sweden +46-8-517 721 35 (office) E-mail: Teresita.Diaz.de.Stahl@ki.se<mailto:teresita.diaz.de.stahl@ki.se>
galaxy • 727 views
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modified 6.6 years ago by Jennifer Hillman Jackson ♦ 25k • written 6.6 years ago by Teresita Diaz De Ståhl • 10
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gravatar for Jennifer Hillman Jackson
6.6 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:
Hello Teresita, Yes, the tool is at the public server under "NGS: SAM Tools -> rmdup remove PCR duplicates". Instructions for use are in the tool form under "What it does". An uploaded BAM file (only upload the .bam, not the index .bam.bai, Galaxy creates that new) will appear on the "Get Data -> Upload File" tool form, in section "Files uploaded via FTP:". From there, check the file(s) and move into a history. Exact instructions, including a screencast, are in the Galaxy wiki: http://wiki.g2.bx.psu.edu/FTPUpload Your FTP client (unix or desktop) will provide information about the status of a transfer. There is a 50G size limit on individual files. Files loaded successfully need to be moved from the "Upload File" transfer area to a history within 3 days (after that they are purged). Hopefully this helps, but please provide more details if you are still having problems, Jen Galaxy team -- Jennifer Jackson http://galaxyproject.org
ADD COMMENT • link written 6.6 years ago by Jennifer Hillman Jackson ♦ 25k
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