Hello I am trying to run my fasta output file from Unique.Seqs with Align.Seqs and I am trying to assign my Silva reference but all it says is no options available and the only reference template I can select is Cashed reference or your history. What might I be doing wrong? Thank you
Hello,
How to retrieve the tutorial data for https://galaxyproject.github.io/training-material/topics/metagenomics/tutorials/mothur-miseq-sop/tutorial.html is explained in the section titled Importing the data into Galaxy.
Specifically, the reference.data.zip archive contains the fasta data used in the tutorial: https://zenodo.org/record/165147/files/reference_data.zip
FAQ: https://galaxyproject.org/support/loading-data/
Hope that helps! Jen, Galaxy team
Oh nuts Jen,
I totally missed that. I am working on my own data with this SOP and skimmed right past that because my sample data was already imported. Sorry to bother you for something so easy to fix. Thank you for your help and immediate response!! much appreciated
Julie
can you also analyze 18s MiSeq runs with this protocol?
ok me again so I figured out that I need to upload my own silva fasta file. I cannot find this silva.v4.fasta file that this protocol 16S Microbial Analysis with Mothur is asking for. I uploaded the most recent release from the silva website but that does not seem to be working...unless i messed up somewhere in the earlier steps. if someone knows the secret can you please let me in on it? I am a new user if you didn't pick up on that already...just trying to find my way. Thank you!!