Latest
Open
RNA-Seq
ChIP-Seq
SNP
Assembly
Forum
Planet
All »
Home
Log In
Question: aaChanges Errors
0
gravatar for richard.coletta
2.9 years ago by
richard.coletta • 0
richard.coletta • 0 wrote:

When I try to run aaChanges  I get the following error, Sequences are not currently available for the specified build.  I am using GRch37.p10 as my reference.  What am I doing wrong?
software error • 647 views
ADD COMMENT • link •
modified 2.9 years ago by Jennifer Hillman Jackson ♦ 25k • written 2.9 years ago by richard.coletta • 0
0
gravatar for Jennifer Hillman Jackson
2.9 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

That human update build is not available on the public Main Galaxy instance at http://usegalaxy.org (or in the default included builds in Cloudman). Using hg19 or hg38 instead is the quickest alternative. This one of the few tools in Galaxy that does not support Custom reference genomes from the history.

However - it might accept a Custom build - I have not tested that usage with this tool, but you could. This wiki link below explains how to promote a custom reference genome to a custom build. Once created, assign the build to all of the input datasets as the "database" attribute.

Should that fail, then setting up a local or cloud Galaxy and adding this genome is the solution. Data managers can be used, so all can be done through the user interface as admin on your own Galaxy (there are no special indexes for this particular tool, just the installed/added genome is utilized, but you could add in indexes for other tools if you wanted).

https://wiki.galaxyproject.org/BigPicture/Choices

https://wiki.galaxyproject.org/Learn/CustomGenomes

https://wiki.galaxyproject.org/Admin/Tools/DataManagers

Take care, Jen, Galaxy team
ADD COMMENT • link written 2.9 years ago by Jennifer Hillman Jackson ♦ 25k
Thanks, using hg19 worked helped!
ADD REPLY • link written 2.9 years ago by richard.coletta • 0

Super, thanks for letting us know! Jen

ADD REPLY • link written 2.9 years ago by Jennifer Hillman Jackson ♦ 25k
Please log in to add an answer.

Similar posts • Search »

Content
Help
Access
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 171 users visited in the last hour