Question: How to put 2 reference choromosomes in one file so that i can perform alignment based on only these 2?
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gravatar for kuick77
2.5 years ago by
kuick77 • 10
kuick77 • 10 wrote:

Hi There,

Just a quick question, I am trying to perform alignment for three of my target genes located in chr 2 and chr3. I uploaded these two reference genome separately. I realised the alignment only allow me to put one reference per analysis. So i created two BAM files from one dataset. I have to load two BAM files to view in IGV. Is there a better way? or how to combine two reference chromosome?

alignment • 590 views
ADD COMMENT • link • written 2.5 years ago by kuick77 • 10

I just use the h19 genome reference instead, though it takes slower.

THanks

ADD REPLY • link written 2.5 years ago by kuick77 • 10
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