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• Metagenomic Workflow
  Hi, I am trying to analyse my eukaryotic metagenome data using yours workflow for windshield spl...
• reads per million (rpm) tool
  Which tools are suitable for: 1. Counting the occurrence of each unique sequence (often referred...
• Problems with the annotation tool on cufflinks
  Once I select the tab to have a annotation reference, the little link doesn't open and I can not ...
• Low alignment for paired end reads using HISAT2
  Hello, I am new to processing and analysis of RNAseq data. I have recently completed a paired-en...
• genome of interest is not listed
  Dear Galaxy team and Galaxy community, I reply on the notice found in HISAT: "If your genome of ...
• Problems converting a file to FASTA
  Hello! How are you? I am having a hard time converting a file. I have  FASTA formatted sequences...
• SnpEff (Galaxy) troubleshoot
  I have got Mpileup data. I wish to further understand SNP effect using snpeff. Unfortunately, in ...
• VERY new to Galaxy: How do I query by row and keep rows with specific values
  Hi - I've been using Galaxy for about a week now on my own. I'm a total newcomer this world, so p...
• De novo transcriptome assembly and reference guided transcriptome assembly
  Hi, I have four related questions about de novo RNAseq data analysis. I have 4 RNAseq data obtai...
• Subtract
  Hello, I am using the subtract (whole dataset) tool. I converted my fastq file to tabular with 2...
• find lowest diagnostic rank
  when we make a metagenomic analysis for a certain samples, during analysis we have a table compo...
• Problem Report!!
  Dear Galaxy member, I'm sending you this e-mail because of a problem I have in fetching sequence...
• Trimmomatic and adapters
  Hi all, As relatively new within the bioinformatics world, I am a bit confused when it comes to ...
• Loading VCF into a local IGV fails with metadata error during data prep step
  Although my genome sequence is part of the list of options thanks to custom build, the link to di...
• Fwd: Exome Sequencing Analysis
  Hello, My name is Johnathan Cooper-Knock, I am a clinical fellow based at the University of Shef...
• Issue With Saving 'Manipulate Fastq' In Workflow; And Request For Advice Dealing With Barcoded 454 Data
  Hi, I'm a new user, learning how to use Galaxy while I wait for my 454 results. So I'm not actua...
• To determine frequency of crispr cas induced indels in bam files
  I have used CRISPR/Cas molecules to target a specific gene. I want to quantify the frequency of i...
• Human RNA-Seq paired-end libraries
  Hello, I would like to study and doing exersizes in RNA-seq analysis that are based on paired-en...