Question: To determine frequency of crispr cas induced indels in bam files
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3.2 years ago by
dr.ks.iitkgp0 wrote:

I have used CRISPR/Cas molecules to target a specific gene. I want to quantify the frequency of indels as a result of targeting by CRISPR/Cas using galaxy or any other easier tool. I already have my bam files ready. Now, I need to align and find frequency of indels in these BAM files. Can anyone give me steps to find frequency of indels using Bam files? Thanks.

galaxy • 1.1k views
ADD COMMENTlink modified 3.2 years ago by Bjoern Gruening5.1k • written 3.2 years ago by dr.ks.iitkgp0
gravatar for Bjoern Gruening
3.2 years ago by
Bjoern Gruening5.1k
Bjoern Gruening5.1k wrote:

This is probably not a good answer but are you aware of the CRISPR RECOGNITION TOOL?

We have a Galaxy wrapper for it, maybe this helps you?

ADD COMMENTlink written 3.2 years ago by Bjoern Gruening5.1k
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