Latest
Open
RNA-Seq
ChIP-Seq
SNP
Assembly
Forum
Planet
All »
Home
Log In
Question: To determine frequency of crispr cas induced indels in bam files
0
gravatar for dr.ks.iitkgp
3.2 years ago by
dr.ks.iitkgp • 0
Belgium
dr.ks.iitkgp • 0 wrote:

I have used CRISPR/Cas molecules to target a specific gene. I want to quantify the frequency of indels as a result of targeting by CRISPR/Cas using galaxy or any other easier tool. I already have my bam files ready. Now, I need to align and find frequency of indels in these BAM files. Can anyone give me steps to find frequency of indels using Bam files? Thanks.
galaxy • 1.1k views
ADD COMMENT • link •
modified 3.2 years ago by Bjoern Gruening ♦ 5.1k • written 3.2 years ago by dr.ks.iitkgp • 0
1
gravatar for Bjoern Gruening
3.2 years ago by
Bjoern Gruening ♦ 5.1k
Germany
Bjoern Gruening ♦ 5.1k wrote:

This is probably not a good answer but are you aware of the CRISPR RECOGNITION TOOL?

We have a Galaxy wrapper for it, maybe this helps you? https://github.com/bgruening/galaxytools/tree/master/tools/crt
ADD COMMENT • link written 3.2 years ago by Bjoern Gruening ♦ 5.1k
Please log in to add an answer.

Similar posts • Search »

Content
Help
Access
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 175 users visited in the last hour