Question: (Closed) Trimmomatic and adapters
gravatar for binibe
21 months ago by
binibe70 wrote:

Hi all,

As relatively new within the bioinformatics world, I am a bit confused when it comes to adapter trimming - and want to get a better understanding. At the moment I am working with PE reads generated on a NextSeq 500 - and I want to use Trimmomatic to remove adapter sequences and low quality reads.

Under the IlluminaClip, there are following options:

TruSeq2 (SE, for Illumina GAII) TruSeq3 (SE, for MiSeq and HiSeq) TruSeq2 (PE, for Illumina GAII) TrueSeq3 (PE, for MiSeq and HiSeq) TruSeq3 (additional seqs) (PE for Miseq and HiSeq) Nextera (PE)

So far I have been using TruSeq3 (additional seqs), since it seemed like the most likely option - but I am not completely confident that it is right. I hope some experienced users maybe can help me out - I want to get it right and understand why I am choosing as I am.

Thank you!

rna-seq galaxy trimmomatic • 2.2k views
ADD COMMENTlink written 21 months ago by binibe70

Hello binibe!

Questions similar to yours can already be found at:

We have closed your question to allow us to keep similar content in the same thread.

If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.


ADD REPLYlink written 8 months ago by Jennifer Hillman Jackson25k
Please log in to add an answer.
The thread is closed. No new answers may be added.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 166 users visited in the last hour