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Question: Mothur make-contigs missing dependencies
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gravatar for jhackman
14 months ago by
jhackman • 0
jhackman • 0 wrote:

So, this is my first time using Mothur on Galaxy. I am following the protocol https://galaxyproject.github.io/training-material/topics/metagenomics/tutorials/mothur-miseq-sop/tutorial.html

I am currently stuck at the 3rd step in the process (quality control)

when I make it to the make.contig section the program will not use my paired reads from the previous step, it keeps defaulting to a pair from the Un-paired data and giving me the error message:

Fatal error: Exit code 127 () mothur: error while loading shared libraries: libboost_iostreams.so.1.57.0: cannot open shared object file: No such file or directory mothur: error while loading shared libraries: libboost_iostreams.so.1.57.0: cannot open sh

I feel as if I am missing a step. If someone could run through the tutorial and see if it truly is a bug or if it is something i am doing it would be greatly appreciated.

admin mothur config usegalaxy.org metagenomics • 492 views
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modified 14 months ago by Jennifer Hillman Jackson ♦ 25k • written 14 months ago by jhackman • 0

Where are you executing the tutorial? Galaxy main (https://usegalaxy.org) or ??

ADD REPLY • link written 14 months ago by Jennifer Hillman Jackson ♦ 25k

I'm executing the tutorial in the main Galaxy page using the NGS: Mothur tool set.

ADD REPLY • link written 14 months ago by jhackman • 0
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gravatar for Jennifer Hillman Jackson
14 months ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

There is now a tracked known issue with the dependencies for some of the Mothur tools. Please follow progress on the correction here: https://github.com/galaxyproject/usegalaxy-playbook/issues/37

Thank you for reporting the problem! Jen, Galaxy team
ADD COMMENT • link written 14 months ago by Jennifer Hillman Jackson ♦ 25k

Update: This was just fixed. Please give it a try now

ADD REPLY • link written 14 months ago by Jennifer Hillman Jackson ♦ 25k
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