Question: Bowtie2/Samtools files are not visualized with Trackster: Fatal error Exit code 255 ()
gravatar for jrzupan
6 weeks ago by
jrzupan0 wrote:

I am trying to map 8 paired-end reads on to the same reference custom-build genome. Each of these mappings produces a Bowtie2 file which can then be used to generate a Samtools file. All 8 of the Bowtie2 files and 8 Samtools files can be displayed on Of the 8 Bowtie files and 8 Samtools files only 3 can visualized with Trackster- these are the Bowtie2 and SAM files from one of the mappings and the SAM (but not the Bowtie2) file from another. 7 Bowtie2 files and 6 SAM files give me the following error message:

Fatal error: Exit code 255 () Couldn't open /cvmfs/ , No such file or directory

Any ideas how I can fix this? Thanks

ADD COMMENTlink modified 6 weeks ago by Jennifer Hillman Jackson25k • written 6 weeks ago by jrzupan0
gravatar for Jennifer Hillman Jackson
6 weeks ago by
United States
Jennifer Hillman Jackson25k wrote:


Check to see if the database is assigned for these datasets. My guess is that it is not and instead, a placeholder of "?" is assigned.

The database must match the genome/build of the genome used in Trackster. This can be a built-in genome build or a custom genome that has been promoted to a custom build.

These FAQs should help:

Please let us know if that does not resolve the issues. Thanks! Jen, Galaxy team

ADD COMMENTlink written 6 weeks ago by Jennifer Hillman Jackson25k
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