Question: align.seq mothur error
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rizkyana.avissa • 0 wrote:
Hi. i am new to galaxy. i'm trying to align my NGS samples based on galaxy 16s Microbial Analysis with Mothur tutorial, my sample use V1-V2 region, so i thought it would be wrong if i use silva.v4.fasta as the reference. i went to silva website, download a reference file and upload it to galaxy, which is SILVA_132_SSURef_Nr99_tax_silva.fasta .
when i try to align my unique.seqs with the reference using align.seq, all parameter default, it comes to an error with message "term environment variable not set". i am trying to check the error details, but i get no other explanation in bug report. it only show this :
Dataset Error
An error occured while running the tool toolshed.g2.bx.psu.edu/repos/iuc/mothur_align_seqs/mothur_align_seqs/1.39.5.0
Tool execution generated the following messages:
Fatal error: Exit code 1 ()
TERM environment variable not set.
TERM environment variable not set
what might i do wrong? should the reference and the sample data be in approximately same length?
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modified 8 weeks ago
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Jennifer Hillman Jackson ♦ 25k
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written
9 weeks ago by
rizkyana.avissa • 0
For reference, the tutorial link: