Question: Splitting pair end file
0
gravatar for jramo033
4 months ago by
jramo0330
jramo0330 wrote:

This is the type of file for pair end DNA sequence I have:

@MONK:275:C1Y6RACXX:1:1101:1169:1943/1 
NAATAAATACTCTAAGACAAATCTATCACTTTAAAAGAGAGAAATCATATTCTAGGTTTTCATTAATGTCAGACTTAGCAGTGAAGATTCTCAGGTTCCT
+
#4=DFFFFHHHHHJJJJJJIIIJJJJJIJJJJIJJJJIJGJGIJJJJIIJJJJIJJHIJJJJJJJIIJIGIIIJJHIIFIJHHHHHHFFFFFFFEEEEED 
@MONK:275:C1Y6RACXX:1:1101:1169:1943/2 
CAAATAAATACTCTAAGACAAAACTTGAATATAGAAAGTTGTAGAGGGTTTTTAGAAATTGATTTATAATACTTACAAATGATGAGTCTGAAAGAAGCCAAG
+ 
CB@@DDFFFHHHHHJIIIJJJJJJJJJFHJGIHHHGIHGHIHIHHIHJ?GHDGHIIIJIJJIIJIJJIIJJIJIJIJIIHFHHGEFCEFFFFEEDEEDDCDA

I need help to split the file in right and reverse reading. The number of reading is not the same (100 vs 102) so I was thinking in using Manipulate FASTQ. Can you help me how to write the code so that at the end I can have two separate files?

fastq paired split • 181 views
ADD COMMENTlink modified 4 months ago by Jennifer Hillman Jackson23k • written 4 months ago by jramo0330
0
gravatar for Jennifer Hillman Jackson
4 months ago by
United States
Jennifer Hillman Jackson23k wrote:

Hello,

Please see this FAQ for how to split the data:

Thanks! Jen, Galaxy team

ADD COMMENTlink modified 4 months ago • written 4 months ago by Jennifer Hillman Jackson23k
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