Question: Making paired-end reads the same length
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mtp1v12 • 0 wrote:
I have paired-end data (in two separate files). I have groomed my files, and would like to filter the reads for quality. However, the read lengths for some of the paired reads are not equal. If I filter the files independently, the mapping fails as the two files contain different numbers of reads. If I use FASTQ Joiner to merge the data, then filter, I cannot use FASTQ Splitter as some reads (~44%) can't be split due to unequal read lengths. Any help gratefully received. For example, is there a way to trim reads so that the paired reads are the same length? I should say that I am a Galaxy newcomer, so go easy...!
