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Du, Jianguang • 380 wrote:
I am new to the NGS analysis. I need help to solve this problem.
As shown in my previous emial/question shown below, I have some
paired-end datasets at FASTQ format, and I have problem to split each
of these datasets into two datasets (one forward and one reverse).
Jennifer instructed me to assign the datatype to be fastqsanger first
and then run 'Manipulate FASTQ'.
I have two questions:
1) Now that the datasets were already split into forward and reverse
reads when extracted in FASTQ format from the SRA, can I use them just
as single end data?
2) If I do need to split each dataset into two datasets, how should I
choose the settings when I run "Manipulte FASTQ"?
Thanks.
Jianguang
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