Question: Can I Convert Paired-End Datasets Into Single End Ones?
1
Du, Jianguang • 380 wrote:
Dear All,
I have some paired-end datasets to be analyzed, but I am not sure
about their Mean Inner Distance between Mate Pairs.
Can I convert these paired-end datasets into single-end ones and use
them as single-end dataset as follows?
1) Use the tool "Manipulate FASTQ" to convert the sequence of reverse
reads into its reverse-complement counter part, so that all of the
reverse reads actually become forward reads.
2) run Tophat on the manipulated datasets as single-end ones.
Thanks.
Jianguang
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modified 6.3 years ago
by
Jennifer Hillman Jackson ♦ 25k
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written
6.3 years ago by
Du, Jianguang • 380
