Question: Splitting Biased Paired End FASTQ data
gravatar for lukacdm
4.5 years ago by
United States
lukacdm0 wrote:

The FASTQ splitter in Galaxy warns that the tool only works if Paired-end data has equal length read ends.  Does anyone know of a Galaxy tool that will correctly split biased paired end data?  I have paired end data with read ends of 50 and 25 nt.  The FASTQ splitter does split the file, but the split files both contain 25 nt ends, so the tool is truncating the longer read ends.

galaxy • 1.4k views
ADD COMMENTlink modified 4.5 years ago • written 4.5 years ago by lukacdm0

Unfortunately, I am not running my own instance of Galaxy.

ADD REPLYlink written 4.5 years ago by lukacdm0
gravatar for Wolfgang Maier
4.5 years ago by
Wolfgang Maier600 wrote:

Maybe someone else knows of an existing tool, but if not, are you running your own instance of Galaxy ? In that case it is not too complicated to add the functionality by modifying FASTQ splitter.

ADD COMMENTlink written 4.5 years ago by Wolfgang Maier600
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