Question: Splitting Biased Paired End FASTQ data
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gravatar for lukacdm
4.5 years ago by
lukacdm • 0
United States
lukacdm • 0 wrote:

The FASTQ splitter in Galaxy warns that the tool only works if Paired-end data has equal length read ends.  Does anyone know of a Galaxy tool that will correctly split biased paired end data?  I have paired end data with read ends of 50 and 25 nt.  The FASTQ splitter does split the file, but the split files both contain 25 nt ends, so the tool is truncating the longer read ends.

galaxy • 1.4k views
ADD COMMENT • link • modified 4.5 years ago • written 4.5 years ago by lukacdm • 0

Unfortunately, I am not running my own instance of Galaxy.

ADD REPLY • link written 4.5 years ago by lukacdm • 0
0
gravatar for Wolfgang Maier
4.5 years ago by
Germany
Wolfgang Maier • 600 wrote:

Maybe someone else knows of an existing tool, but if not, are you running your own instance of Galaxy ? In that case it is not too complicated to add the functionality by modifying FASTQ splitter.

ADD COMMENT • link written 4.5 years ago by Wolfgang Maier • 600
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