Question: RNAseq analysis starter
0
bassam.abomoelak • 10 wrote:
I just received my data for RNAseq and I appreciate guiding me through the steps of analysis. Thanks
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4
Mo Heydarian ♦ 830 wrote:
Hello,
Have a look at the tutorials on transcriptomics from the Galaxy Training Network: http://galaxyproject.github.io/training-material/topics/transcriptomics/
There are two tutorials there, one for reference based RNA-seq analysis and another for de novo transcriptome reconstruction RNA-seq analysis. All of the tools in the latter can be used on useGalaxy.org .
These tutorials have step-by-step instructions for running the tools and screenshots to guide you along the way.
Best of luck with your analysis.
Cheers,
Mo Heydarian
0
bassam.abomoelak • 10 wrote:
Dear Mo, Thanks so much for your reply. I will start soon navigating the system. Bassam
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bassam.abomoelak • 10 wrote:
some of my fastq files are above 7GB. I know I can't upload from my computer. Is pasting the files link easier than FTP route?. if so, can I transfer them as folder or one by one?. Thanks for help
bassam
0
Mo Heydarian ♦ 830 wrote:
Hi Bassam,
If you have links to the files you can add them with the "Paste/Fetch data" option in the upload portal. If you would like help setting up a FTP connection, please see this guide: https://galaxyproject.org/ftp-upload/
Thanks for using Galaxy!
Cheers,
Mo Heydarian
0
bassam.abomoelak • 10 wrote:
My RNAseq files in txt.bZ2 format. Is there anyway to convert them into fastq.gz?. Thanks
Bassam
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