Preparing for trinity analysis I try to trim questionable 5' bases. Trying to use 'Trimmomatic', I can't load my data set that was analyzed with fastqc. My files show up/ are accessible in other routines.
Hello,
These are fastq datasets? They might be too large to load with the "Browse for local file" option. Try FTP instead. This is how: https://wiki.galaxyproject.org/Support#Loading_data
Thanks, Jen, Galaxy team
Hi Jennifer,
Thanks for the response. I do have my data files (uploaded as you suggested with FTP). They do show up in some routines but not in the ones I need.
KHH
From: Jennifer Hillman Jackson on Galaxy Biostar [mailto:notifications@biostars.org] Sent: Wednesday, November 02, 2016 2:34 PM To: khh@louisiana.edu Subject: [BULK] [galaxy-biostar] unable to input file in trimmomatic Importance: Low
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User Jennifer Hillman Jackson http://biostar.usegalaxy.org/u/254/ wrote Answer: unable to input file in trimmomatic http://biostar.usegalaxy.org/p/20384/#20386 :
Hello,
These are fastq datasets? They might be too large to load with the "Browse for local file" option. Try FTP instead. This is how: https://wiki.galaxyproject.org/Support#Loading_data
Thanks, Jen, Galaxy team
You may reply via email or visit http://biostar.usegalaxy.org/p/20384/#20386
By routines do you mean tool forms? If so, the problem could be the datatype assignment. Some tools will require just fastq and others will require fastqsanger.
https://wiki.galaxyproject.org/Support#Tool_doesn.27t_recognize_dataset
Thank you - it was the wrong data format. Unfortunately the required FASTQ Groomer is under ‘NGS: QC and manipulation’ not under ‘Convert Formats’.
KHH
From: Jennifer Hillman Jackson on Galaxy Biostar [mailto:notifications@biostars.org] Sent: Wednesday, November 02, 2016 3:59 PM To: khh@louisiana.edu Subject: [galaxy-biostar] unable to input file in trimmomatic
Activity on a post you are following on Galaxy Biostar http://biostar.usegalaxy.org
User Jennifer Hillman Jackson http://biostar.usegalaxy.org/u/254/ wrote Comment: unable to input file in trimmomatic http://biostar.usegalaxy.org/p/20384/#20389 :
By routines do you mean tool forms? If so, the problem could be the datatype assignment. Some tools will require just fastq and others will require fastqsanger.
https://wiki.galaxyproject.org/Support#Tool_doesn.27t_recognize_dataset
https://wiki.galaxyproject.org/Support#FASTQ_Datatype_QA
You may reply via email or visit http://biostar.usegalaxy.org/p/20384/#20389