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• Trimmomatic data on Fastqc
  Hell, i am unable to load "Trimmomatic on Fastq groomer on data file"on Fastqc. it show Fatal err...
• Trimmomatic and Trim Galore are not working.
  I am performing an RNA-seq and am trying to trim adapter sequences with Trim Galore. When I made ...
• Cannot select fastq data in Galaxy Trimmomatic
  Hi Galaxy-verse I am trying to open my uploaded paired-end MiSeq reads in Trimmomatic from withi...
• HTSEQ count error
  Hello, This is my first rna-seq data analysis project and I am using Galaxy to analyze different...
• Fatal error in Trimmomatic
  Hi all, I have been trying to run a Trimmomatic trim on my data since Friday. Trimmomatic will b...
• Illumina Clip Step in Trimmomatic
  In trimmomatic, under Perform Initial Illumina Clip step, which adapter sequence should I specify...
• Trimmomatic works good?
  Hi, I am newbie in data analysis of RNAseq. I have tried to remove the illumna adapters by trimmo...
• NGS: QC and manipulation
  Hello, I'm a beginner (my email zare@ibt.lt) of using Galaxy. I tried to use Trimmomatic or Trim ...
• Running Trimmomatic on loop for Paired End
  I'm need to do trimming for Paired End. Is there any way around where I can run the trimmomatic ...
• unable to input file in trimmomatic
  Preparing for trinity analysis I try to trim questionable 5' bases. Trying to use 'Trimmomatic', ...
• Galaxy-using trimmomatics with ChIP seq pair ended (only one file with both reads)
  I am trying to run Trimmomatics in Galaxy under "pair end (as collection)" for afastqsanger.gz ...
• Local Galaxy Sending Job Error
  Hi, I am relatively new to Galaxy. I am using Galaxy as a local. I installed tools via admin Tool...
• miRNA seq data TRIMMOMATIC
  I am trying to analyze microRNA sequencing data for 8 datasets ( each dataset is a cell line). I ...
• Nextera pair end adapter cleaning
  Can I use Nextera pair end adapter option in Galaxy Trimmomatic program for cleaning my illumina ...
• Trimmomatic output after filtering paired end data - fastqsanger vs fastqsanger.gz
  After running Trimmomatic on a paired end PolyA RNA-seq dataset where both forward and reverse re...
• trimmomatic with paired-end
  Dear Biostar, I have paired-end sequencing data I would like to trim using trimmomatic. I have r...
• Paired end illumina RNAseq reads - running trinity with paired trimmed files and adding unpaired as single read?
  We have paired end Illumina HiSeq 4000 reads that we are working to remove adaptors and trim base...
• GROOMER ON NEXTSEQ500 DATA
  Dear community, I am a beginner....trying to use Trimmomatic to clean adaptors/index from my Nex...
• Trinity run preprocessing
  I am new to Trinity and galaxy instance (https://usegalaxy.org/). I have 17 samples pair ended. I...
• RNAseq data to be processed in two ways: (i) mapping to de novo Trinity-based transcriptome and (ii) mapping a relatively new genome
  Hello all, I am new to RNAseq data and learning this process step by step, so I have a few quest...
• Error with Unicycler at Galaxy Main https://usegalaxy.org
  Dear Biostars Members, I shall kindly ask a question considering bacterial genome assembly with ...
• Cuff diff output vs genotype discrepancy
  Hi there, I am using tuxedo pipeline to analyse differential gene expression between my samples....