Question: Please add reference genome to Bowtie 2
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gravatar for jkherman
3.2 years ago by
jkherman • 0
United States
jkherman • 0 wrote:

I would like to map my reads to a reference genome that is not available in the pull-down menu of Bowtie 2.

 

It says to "contact the galaxy team"

 

The reference genome I am using is:

 

Bacillus subtilis subsp. subtilis str. 168 chromosome

http://www.ncbi.nlm.nih.gov/nuccore/NC_000964.3  (Refseq)

http://www.ncbi.nlm.nih.gov/nuccore/255767013?report=genbank

-Jen Herman

bowtie galaxy • 691 views
ADD COMMENT • link • modified 3.2 years ago by Jennifer Hillman Jackson ♦ 25k • written 3.2 years ago by jkherman • 0
0
gravatar for Jennifer Hillman Jackson
3.2 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Use the genome as a Custom Reference Genome/Build for access to it right now. Instructions are in the Galaxy Support wiki, Section 2.15
https://wiki.galaxyproject.org/Support

Smaller genomes such as yours work very well with this method.

Best, Jen, Galaxy team

ADD COMMENT • link written 3.2 years ago by Jennifer Hillman Jackson ♦ 25k
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