Question: Add a reference genome
gravatar for lidong460
21 months ago by
lidong4600 wrote:

Dear Mr./Ms., I just want to use HISAT in RNA-Seq, and I want to use Pseudomonas aeruginosa PAO1 as a reference genome, while it is not in the reference genome list. Can we add it in the reference genome list? I think we can get the whole genome of this bacterial strain from this link Thank you very much!


rna-seq • 578 views
ADD COMMENTlink modified 21 months ago by Jennifer Hillman Jackson25k • written 21 months ago by lidong4600
gravatar for Jennifer Hillman Jackson
21 months ago by
United States
Jennifer Hillman Jackson25k wrote:


Use this genome in fasta format in Galaxy as a Custom Reference Genome/Build. Run the tool NormalizeFasta as the first step after uploading to ensure proper formatting and to reduce problems that can occur with toosl when the fasta format is not standardized. This is how:

Thanks, Jen, Galaxy team

ADD COMMENTlink written 21 months ago by Jennifer Hillman Jackson25k
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