Question: Should Fastq Files Be Merged?
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Karen Margrethe Jessen • 20 wrote:
Hi,
I have downloaded the fastq.tgz files for an ENCODE RNA-SEQ data and
unpacked the files. The data set is paired end illumina. I am a bit
confused, as there are 5 files for read1 and 5 files for read2 for
each sample. Am I supposed to merge the 5 files before aligning to the
hg19 genome?
If yes, how should I merge these files?
I would greatly appreciate any help you can provide.
Best regards,
Karen Margrethe Jessen
Cand. Scient., ph.d.-student
Aarhus University
Denmark
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modified 6.0 years ago
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Jennifer Hillman Jackson ♦ 25k
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written
6.0 years ago by
Karen Margrethe Jessen • 20