Question: Merging Of Illumina Paired End Files
gravatar for Arun Khattri
7.2 years ago by
Arun Khattri10
Arun Khattri10 wrote:
I have 3 illumina paired end reads of exome capture of the sample. I want to assemble these reads to genome using tools available in Galaxy (BWA etc). My concern is the amount of data that I can analyzed and when these reads should be merged. The total size of data is +30Gb. Thanks, Arun
bwa alignment • 2.8k views
ADD COMMENTlink modified 7.2 years ago by Jennifer Hillman Jackson25k • written 7.2 years ago by Arun Khattri10
gravatar for Jennifer Hillman Jackson
7.2 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello Arun, In brief, you will want to load your data via FTP, groom the files, and run BWA for Illumina with the paired-end data option (for mapping, not assembly). Help to get started can be found at: An initial input of 30G would likely not be a problem, but it depends on what analysis steps you intend to do after mapping. A Cloudman option may be more suitable, please see: Hopefully this helps, Best, Jen Galaxy team -- Jennifer Jackson
ADD COMMENTlink written 7.2 years ago by Jennifer Hillman Jackson25k
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