Question: Should Fastq Files Be Merged?
5.9 years ago by
Karen Margrethe Jessen • 20 wrote:
Hi, I have downloaded the fastq.tgz files for an ENCODE RNA-SEQ data and unpacked the files. The data set is paired end illumina. I am a bit confused, as there are 5 files for read1 and 5 files for read2 for each sample. Am I supposed to merge the 5 files before aligning to the hg19 genome? If yes, how should I merge these files? I would greatly appreciate any help you can provide. Best regards, Karen Margrethe Jessen Cand. Scient., ph.d.-student Aarhus University Denmark
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