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• Nextera pair end adapter cleaning
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• Sample For Video For Example 3 In Ngs Analyses In Galaxy
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• Fastq Quality Trimmer For Pe Illumina?
  Hello, I was wondering, can Galaxy's FASTQ Quality Trimmer tool be used on Illumina paired-end d...
• Merging Of Illumina Paired End Files
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• trimmomatic with paired-end
  Dear Biostar, I have paired-end sequencing data I would like to trim using trimmomatic. I have r...
• Reconstruct original fragment from paired end reads
  Hi, I would like to reconstruct my environemental 16S PCR fragments (slightly more than 300 bp) ...
• difference between tophat2 and tophat for illumina
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• Best Tophat parameters for splicing
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• Pre-Processing Of Illumina Rna-Seq Paired End Data
  Hello, I have Illumina 76bp paired end data for a zebrafish RNA-seq experiment and am basically ...
• De-novo assembly of a bacterial genome
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• RNA-seq data analysis workflow!
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• Illumina HiSeq reads and Fastq joiner
   Hello, I have paired-end reads (Illumina HiSeq) from a metagenome in two Fastq files. These non...
• Initial Qc And Grooming For Illumina Hiseq2000 Paired End Rnaseq Data
  I am trying to do RNAseq analysis on Paired end data from the Hiseq2000. I have about 50 files f...
• How to set 'mean inner distance' for paired-end reads?
  Hi all, If I use Paired-end sequences for 'Tophat for illumina' or 'Tophat 2', I need to type in...
• Trimmomatic on paiera end data
  Hi I am trying to use Trimmomatic on my Sanger Illumina 1.9 paired end data. Trimmomatic ask fo...
• Kraken (assign taxonomic label...)
  One Illumina file fastq.gz contain all info about paired-end reads. I used by usegalaxy interface...
• Barcode Splitter On Paired End Reads
  Dear Galaxy team,   I am sorry for repeatedly asking this question but I would like some feed bac...
• Paired end illumina RNAseq reads - running trinity with paired trimmed files and adding unpaired as single read?
  We have paired end Illumina HiSeq 4000 reads that we are working to remove adaptors and trim base...
• Can someone give me a foolproof instruction on how to merge R1 and R2 illumina fastq files
  Dear Galaxy experts,               I need to merge R1 and R2 Illumina paired end reads. T...
• Trimming And Thinning Of Paired-End Reads
  Hi galaxy users, I've been experiencing some problems trimming the adapters and filtering by qua...
• Need help with "Tophat for Illumina" tool
  Dear All, Hello amazing Galaxy team members  now i have 14 manipulated fastq files 7 forward an...
• Bowtie don't load paired end data
  Hi, I'm new in the NGS technology. I have a problem with Bowtie paired end loading data. A few we...
• Filtering Pile-Up Fails
  Dear All, I have been successful by using the online tool to align Illumina pair end reads , eac...
• Forward and Reverse reads from paired end sequencing
  The fastq dataset we have after illumina whole genome sequencing is in the form of two files - fo...