DearXXX, Dear sir/madam I am sorry to bother you. I have a question regarding GALAXY. We are cloning a dominant mutant. The rationale is to collect F2 wild type animals. Once we narrow down to a small region, we can sequence the whole region and identify the related mutation. However, when I use GALAXY, I found that the maximiun of the ratio Hawaii/N2 is only 0.7, and I did not get a reasonale distribution of the ratio. I am wondering if there is a way to reset the Y-axis (set the top as 1), or if you have any other suggestion to deal with this data? Thank you so much for your time and help.
P0: Hawaii X mutant gene ;N2 | F1: mutant heterozygote | F2: WT phenotype