Question: (Closed) inquiry about the analysis of a dominant mutant with GALAXY
gravatar for 17317822349
8 months ago by
1731782234910 wrote:

DearXXX, Dear sir/madam I am sorry to bother you. I have a question regarding GALAXY. We are cloning a dominant mutant. The rationale is to collect F2 wild type animals. Once we narrow down to a small region, we can sequence the whole region and identify the related mutation. However, when I use GALAXY, I found that the maximiun of the ratio Hawaii/N2 is only 0.7, and I did not get a reasonale distribution of the ratio. I am wondering if there is a way to reset the Y-axis (set the top as 1), or if you have any other suggestion to deal with this data? Thank you so much for your time and help.

P0: Hawaii X mutant gene ;N2 | F1: mutant heterozygote | F2: WT phenotype

galaxy • 357 views
ADD COMMENTlink written 8 months ago by 1731782234910

You'll need to specify which particular tools in Galaxy you're using, specifically which one you're using to produce the plot you mention.

ADD REPLYlink written 8 months ago by Devon Ryan1.9k

What i used to produce the plot is exactly the workflow : imported: CloudMap Hawaiian Variant Mapping with WGS and Variant Calling workflow

ADD REPLYlink written 7 months ago by 1731782234910

Hello 17317822349!

Questions similar to yours can already be found at:

We have closed your question to allow us to keep similar content in the same thread.

If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.


PS: Cloudmap has been deprecated. The new tools are now installed at Galaxy Main
ADD REPLYlink written 7 months ago by Jennifer Hillman Jackson25k

Duplicated Q&A with advice from the tool author:

ADD REPLYlink modified 7 months ago • written 7 months ago by Jennifer Hillman Jackson25k
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