Question: (Closed) inquiry about the analysis of a dominant mutant with GALAXY
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17317822349 • 10 wrote:
DearXXX, Dear sir/madam I am sorry to bother you. I have a question regarding GALAXY. We are cloning a dominant mutant. The rationale is to collect F2 wild type animals. Once we narrow down to a small region, we can sequence the whole region and identify the related mutation. However, when I use GALAXY, I found that the maximiun of the ratio Hawaii/N2 is only 0.7, and I did not get a reasonale distribution of the ratio. I am wondering if there is a way to reset the Y-axis (set the top as 1), or if you have any other suggestion to deal with this data? Thank you so much for your time and help.
P0: Hawaii X mutant gene ;N2 | F1: mutant heterozygote | F2: WT phenotype
You'll need to specify which particular tools in Galaxy you're using, specifically which one you're using to produce the plot you mention.
What i used to produce the plot is exactly the workflow : imported: CloudMap Hawaiian Variant Mapping with WGS and Variant Calling workflow
Hello 17317822349!
Questions similar to yours can already be found at:
We have closed your question to allow us to keep similar content in the same thread.
If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.
Cheers!
Duplicated Q&A with advice from the tool author: https://biostar.usegalaxy.org/p/27278